Maryland Venture Fund, established in 1994 and based in Columbia, Maryland, is an early-stage venture capital firm with a focus on nurturing innovative companies within the state. With a capital base of $110 million, the Fund engages in both direct investments in technology and life sciences, as well as indirect investments in venture capital funds. It typically invests between $0.1 million and $1 million in companies that generate revenues up to $1 million, primarily targeting businesses with fewer than 50 employees that have been operational for no more than five years. The Fund specializes in sectors such as healthcare technology, cybersecurity, and information technology. Investments are structured as equity or convertible debt, and the firm seeks to support companies certified as qualified Maryland cybersecurity entities by the state’s Department of Business and Economic Development.

The University System of Maryland Momentum Fund, established in 2016 and based in Baltimore, Maryland, operates as a venture capital firm dedicated to investing in early-stage startups affiliated with the University System of Maryland. With a commitment of $10 million from USM, the fund aims to promote entrepreneurship, attract external investments, and stimulate economic development and technology commercialization within the region. The fund is sector agnostic, focusing on a diverse range of industries while prioritizing startups that are headquartered in Maryland and have connections to USM's member institutions. By leveraging the resources of its affiliated universities, the Momentum Fund seeks to enhance the entrepreneurial ecosystem and support the growth of innovative companies in the state.

Established in 2012 by Christopher College and Stuart Sutley, TCP Venture Capital is a venture capital firm headquartered in Hampstead, Maryland. It focuses on investing in early-stage technology companies based in Baltimore.

New Enterprise Associates is a venture capital firm founded in 1977 and based in Menlo Park, California, with offices in the United States, India and China. It makes investments across technology and healthcare startups at multiple stages, from seed to growth, including software, cloud, data, internet services, fintech, AI, e-commerce, and enterprise technology, as well as healthcare information technology, life sciences, medical devices, and biopharma. It also considers energy technology and infrastructure opportunities, such as solar, batteries and smart grids. The firm pursues a global approach, with activity in the United States, Asia and other regions, and supports companies through rounds of financing ranging from small to sizable investments as they scale. NEA focuses on building portfolios in software, IT services, consumer technology, and healthcare technology, backing ambitious entrepreneurs across sectors and geographies.

Founded in 2014, Gaingels is a venture investment syndicate dedicated to supporting the LGBT+ community. It invests globally in companies led by LGBT+ founders or C-suite executives at all stages of growth, focusing on inclusivity and positive social change.

Grotech Ventures is a Maryland-based venture capital firm founded in 1984 that focuses on early-stage and growth technology companies across the United States. The firm targets sectors including technology, cybersecurity, fintech, supply chain and logistics, software and vertical software, and TMT. It typically makes initial investments in the low to mid single digits per company and reserves capital for follow-on rounds to support growth through the company’s early and expansion stages. Grotech emphasizes a personal, long-term partnership with entrepreneurs, aligning its support with the founder’s definition of success. Beyond capital, it seeks to contribute through its experience, network, and hands-on involvement to help companies mature and scale.

Camden Partners is a United States-based investment firm founded in 1995 and based in the Baltimore, Maryland area. It acts as an asset manager providing growth and private equity capital to lower-middle-market companies, primarily in technology, software, education, healthcare, and business services. The firm pursues early to growth-stage investments across North America and Western Europe, applying sector knowledge and active involvement to help portfolio companies scale. Camden Partners emphasizes disciplined, fundamentals-driven approach and seeks long-term partnerships with management teams and other investors, including board-level engagement where appropriate. The company operates as a registered investment adviser and focuses on building lasting value through strategic capital and advisory support.

DataTribe is a startup foundry based in Fulton, Maryland, established in 2015. The company focuses on investing in and co-building innovative startups that aim to make significant advancements in cybersecurity and data science. Founded by experienced investors and veterans from the U.S. intelligence community, DataTribe leverages its extensive network and professional expertise to provide companies with essential resources, including capital and in-kind services. By fostering collaboration and offering strategic support, DataTribe strives to give its startups a competitive edge in the rapidly evolving tech landscape.

Alumni Ventures Group is a venture capital firm based in Manchester, New Hampshire, with offices across North America. It provides access to venture investing for individual accredited investors, particularly alumni networks, by offering diversified portfolios of venture opportunities and allowing members to invest alongside fellow alumni in ventures led by peers. The firm backs early-stage and later-stage opportunities across technology, consumer, healthcare, financial services, and other sectors, with a sector- and geography-agnostic approach. It typically invests between $10,000 and $3 million per deal and emphasizes the involvement of alumni connections and an institutional lead investor in opportunities. Alumni Ventures also offers focused funds to broaden participation, enabling accredited investors to access venture portfolios diversified by type, sector, stage, and geography. The model aims to democratize access to venture capital while maintaining rigorous selection standards through alumni networks.

Intersouth Partners is a Durham, North Carolina–based venture capital firm founded in 1985 that concentrates on seed and early-stage investments in technology and life sciences. It seeks to be a lead or co-lead investor, often participating in the first institutional round with initial investments in the range of several hundred thousand to several million dollars and pursuing a total investment across rounds around ten to twelve million dollars. The firm emphasizes equity investments and typically seeks a board seat on portfolio companies, aiming to support growth in sectors such as software, semiconductors, communications, digital media, clean technology, security, medical devices, biopharmaceuticals, agricultural biotechnology, and biomaterials. Its geographic focus centers on the Southeast, including the Research Triangle area, Washington, DC, Atlanta, and the broader corridor from Baltimore to Florida. The firm is known for its active engagement and long-standing experience in building early-stage technology and life sciences companies.

Blu Venture Investors is a venture capital investment company based in Vienna, Virginia, founded in 2010. It focuses on seed and early-stage opportunities across technology sectors including software, information technology, cybersecurity, Internet of Things, digital media, materials and chemical/biological sciences, as well as defense, security and intelligence domains. The firm provides initial growth-stage funding combined with active mentorship to help entrepreneurs scale, and it works with experienced operating executives who have launched new companies and products. Blu Venture Investors typically targets early rounds and may participate in syndicates with other investors; it seeks to support ventures with hands-on involvement, including board seats and advisory input when appropriate. The approach emphasizes practical guidance and strategic support to help portfolio companies navigate product development, go-to-market and regulatory considerations in technology-driven industries.

Greenspring Associates, established in 2000, is a global investment firm headquartered in the United States. It specializes in fund of funds management, offering commingled funds and customized partnership solutions to a diverse range of international clients. The firm invests in established and emerging venture capital fund managers, early and growth-stage venture-backed companies, and secondary venture capital fund and direct investments. With over $7.3 billion in committed capital, Greenspring's dedicated team combines extensive expertise in both venture fund and direct investments.

Camber Creek is a venture capital investment firm focused on technology-related opportunities in real estate. Headquartered in Rockville with an office in New York, the firm concentrates on investing in real estate technology companies across the United States. It leverages expertise across real estate sectors such as construction, property management, development, and leasing, and combines investing, operating, and technology know-how with the assets of its partners, investors, and advisors to support the growth and success of its portfolio companies.

Founded in 1989, OrbiMed is a New York-based healthcare-dedicated investment firm managing approximately $5 billion in assets. It invests across the spectrum of healthcare companies, from private start-ups to large multinational corporations, focusing on biopharmaceuticals, life sciences, digital health, medical devices, and diagnostics sectors globally.

Conscious Venture Lab is an early-stage business accelerator based in Baltimore, Maryland, established in 2013. The firm is dedicated to nurturing purpose-driven companies that aim to leverage capitalism for a more just, joyful, and equitable society. It focuses on various sectors, including cybersecurity, biotechnology, technology infrastructure, consumer products, and gaming, among others. Conscious Venture Lab typically invests between $25,000 and $50,000 for equity stakes of 5 to 10 percent. The accelerator runs a six-month program designed for teams with a minimum of three members. The initiative honors the legacy of David B. Wolfe and incorporates insights from leading thinkers in Conscious Capitalism, fostering the development of "Companies that Matter."

Founded in 1997, Novak Biddle Venture Partners is a private equity and venture capital firm based in Bethesda, Maryland. It specializes in seed to early-stage investments in information technology companies, with a focus on the Mid-Atlantic region of the United States.

Founded in 2017, SaaS Ventures is a private equity firm based in Maryland. It focuses on investing in seed-stage technology companies, particularly Software as a Service (SaaS) businesses across North America.

Moonshots Capital is a venture capital firm based in Austin, Texas. It makes seed-stage investments in technology startups led by founders who are building products that re-imagine work and personal life in large and growing markets. The firm uses a disciplined investment process to identify companies with moonshot potential. It focuses on dual-use technology, fintech, consumer Internet, artificial intelligence, and cybersecurity. Moonshots Capital is a registered investment adviser and supports portfolio companies through early growth to scale.

Ben Franklin Technology Partners of Southeastern Pennsylvania is a technology-focused capital provider based in Philadelphia, founded in 1982 to advance the region's technology community. The organization funds and supports technology companies across Pennsylvania, especially in information technology, health, and physical sciences, investing substantial capital in early-stage and established ventures. It has invested over 170 million dollars in more than 1,750 regional technology companies and has launched university-industry partnerships to translate scientific discoveries into commercial products, while seeding regional initiatives that strengthen the entrepreneurial ecosystem and create jobs.

NextGen Venture Partners is a venture capital firm founded in 2011 that focuses on seed and early-stage investments in technology companies across the United States. The firm operates through a broad network of venture partners and targets category-defining and industry-defining technology opportunities in a sector-agnostic approach. It typically invests in seed and early rounds, with check sizes described as ranging from hundreds of thousands to a couple million dollars, and may participate in larger rounds as needed. Headquartered in Baltimore, Maryland, with additional offices in Boston and Arlington, the firm emphasizes building partnerships with founders and accelerating growth through its expansive partner network. NextGen seeks to back ambitious companies using collaborative funding structures and strategic support to help scale globally.

TDF Ventures LLC is a venture capital firm founded in 2004 and headquartered in Chevy Chase, Maryland, with additional offices in Washington, D.C., and Silicon Valley. The firm specializes in early-stage investments, primarily targeting seed stage and Series A companies across various sectors, including infrastructure, software, services, cybersecurity, artificial intelligence, and e-commerce. TDF Ventures typically invests between $0.5 million and $5 million per transaction, focusing on companies that enhance business processes, improve broadband access, or innovate in technology and communication. The firm seeks to engage actively with its portfolio companies and often considers co-investment opportunities. TDF Ventures is committed to supporting startups that address critical industry challenges and leverage emerging technologies to create value in enterprise markets.

301 Ventures is a student-run venture fund based in College Park, Maryland, focused on supporting entrepreneurs affiliated with the University of Maryland and the greater Washington, D.C. area. Established in 2015, the fund manages $500,000 and specializes in making pre-seed investments in companies founded by University of Maryland students and alumni. Its primary aim is to accelerate product development and prepare these startups for future funding opportunities.

Lockheed Martin Ventures, established in 2014, is a venture capital arm of Lockheed Martin Corporation. It focuses on investing in early-stage companies specializing in advanced technologies such as autonomous systems, cybersecurity, space technologies, and artificial intelligence.

Highland Capital Partners is a Massachusetts-headquartered global venture capital firm that invests in seed, early and growth-stage consumer and enterprise technology companies. It maintains offices in Boston, Silicon Valley and Shanghai and has raised substantial capital and backed more than 225 companies across consumer and enterprise technology, supporting entrepreneurs with a focus on building lasting businesses. The firm also runs mentorship initiatives such as Summer@Highland. Its European presence, Highland Europe, founded in 2012, targets rapidly growing internet, mobile and software companies in Europe and works closely with the firm to pursue primary investments as well as acquisition finance or lead secondary transactions.

Sagamore Ventures is an early-stage investment firm located in Baltimore, Maryland, founded in 2014. As a family office, it focuses on investing in various sectors, including real estate, hospitality, and food and beverages. The firm aims to support innovative businesses and foster growth within the Baltimore region. Through its strategic investments, Sagamore Ventures seeks to contribute to the local economy while identifying opportunities that align with its vision for long-term success.

Hibiscus Bioventures is a biotechnology startup studio based in Rockville, Maryland. Founded in 2019, it builds and invests in companies operating in the biotechnology and pharmaceutical sectors.

Red Abbey Venture Partners is a life sciences venture capital firm founded in 2004 and based in Lutherville-Timonium, Maryland. It invests across biotechnology, specialty pharmaceuticals, medical devices, diagnostics, and research tools, targeting opportunities in North America and Europe. The firm supports early-stage and growth-stage companies developing next-generation therapies, devices, and enabling tools, with the aim of generating superior risk-adjusted returns.

Grit Capital Partners is a venture capital firm founded in 2015 and based in New York, with an additional office in Hunt Valley, Maryland. The firm specializes in early-stage investments, focusing on high-potential entrepreneurs who aim to disrupt industries through technology and data. Grit Capital Partners typically invests its own capital and prioritizes companies with at least one technical co-founder. It seeks opportunities primarily in sectors such as financial services, commerce, media, and healthcare information technology. The firm leverages its extensive network of entrepreneurs, executives, and technology veterans to support its portfolio companies and enhance their chances of success.

MedImmune, established in 1988, is a global biopharmaceutical company and the biologics R&D arm of AstraZeneca. It specializes in developing medicines for unmet medical needs, focusing on therapeutic areas such as respiratory, inflammation, autoimmunity, cardiovascular and metabolic disease, oncology, neuroscience, and infection and vaccines. MedImmune Ventures, its venture capital arm founded in 2002, invests in early to late-stage, public and private biotechnology companies globally, with an average investment of $7 million. The firm seeks to invest in companies developing small and large molecule therapeutics, vaccines, pharmaceutical technologies, and platforms, as well as medical devices, diagnostics, and healthcare information technology companies, supporting the discovery, development, and commercialization of pharmaceutical products.

A-Level Capital, LLC is a venture capital firm established in 2015 and located in Baltimore, Maryland. The firm is dedicated to investing in start-up companies founded by students and young alumni of Johns Hopkins University. A-Level Capital primarily focuses on sectors such as medical, technology, and biotechnology, aiming to support innovative ventures emerging from the university community. The firm provides funding opportunities to help these start-ups grow and succeed in their respective markets.

JMI Equity is a Baltimore-based growth equity and private equity firm founded in 1992 that concentrates on software and technology-enabled sectors in North America. It partners with exceptional management teams to fund growth and expansion, helping portfolio companies scale their software, cloud, and TMT businesses into industry leaders. The firm focuses on North American software companies and related tech-enabled services, providing flexible capital and strategic support to accelerate product development, go-to-market execution, and customer growth. Over its history, JMI has raised billions of dollars in committed capital and completed numerous exits, reflecting a track record of building enduring software platform companies.

Eli Lilly and Company is a global pharmaceutical leader engaged in the discovery, development, manufacture, and sale of medicines. It operates facilities in the United States, Puerto Rico, and many other countries, with products sold in about 135 countries. The company focuses on therapies in neuroscience, cardiometabolic, cancer, and immunology, and also conducts research in animal health and animal agriculture. Lilly supports patient outcomes and education through a philanthropic foundation that funds programs in underserved communities, including STEM education in Indianapolis.

Gula Tech Adventures, Inc. is a venture capital firm based in Ellicott City, Maryland, that specializes in investing in early-stage start-ups within the cybersecurity sector. Founded in 2017, the firm focuses on seed and Series A investments, particularly in areas such as cyber hygiene, threat management, security engineering, and national security technology. The founders, Ron and Cyndi Gula, are veterans of the cybersecurity industry, having previously co-founded Tenable Network Security. They leverage their extensive experience to support the next generation of cybersecurity start-ups by providing funding, assisting with product development, and guiding capital raises. Gula Tech Adventures aims to foster innovation and growth in the cybersecurity landscape.

Healthworx is a corporate venture arm based in Baltimore, Maryland, that focuses on enhancing healthcare quality, accessibility, and affordability through innovation and investment. Established in 2017, it operates as part of CareFirst of Maryland, Inc., and is dedicated to supporting and creating companies that aim to transform the healthcare landscape. Healthworx envisions a healthier future for all individuals by fostering advancements in the healthcare sector.

Founded in 2020 by George Petrocheilos and Dr. Jacob Vogelstein, Catalio Capital is a venture capital firm based in Baltimore, Maryland. It specializes in investing in breakthrough biomedical technology companies.

Founded in 2019, Early Light Ventures is a disciplined seed-stage investor focused on B2B software companies. Based in Rockville, Maryland, they prioritize adding value and moving swiftly for the right companies, with a special affinity for those in the mid-Atlantic region.

Squadra Ventures is an early-stage venture capital fund based in Baltimore, Maryland, founded in 2019. The firm focuses on investing in early-stage companies that operate within the cyber and national security sectors. Through its targeted investment strategy, Squadra Ventures aims to support innovative companies addressing critical challenges in these industries.

Inflection Point Ventures is a venture capital firm that concentrates on early-stage technology companies. Based in Newark, Delaware, with additional offices in Wellesley, Massachusetts, and Bethesda, Maryland, it targets pre-revenue and early-revenue firms in telecommunications, information technology, and electronic commerce that show potential for rapid growth and market leadership. The firm seeks opportunities capable of creating an inflection point in demand by delivering high-value products or services. It typically participates in rounds of roughly $500,000 to $2 million and often leads or co-leads investments, taking a seat on the portfolio company's board. By focusing on technology-driven businesses with scalable models, Inflection Point Ventures aims to support companies as they scale, accelerate revenue, and capture market share in competitive markets.

jVen Capital is a venture capital firm that specializes in growth equity investments in life sciences, with emphasis on personalized medicine, medical devices, and digital health. Founded in 2007, it backs companies that aim to advance healthcare through innovative technologies.

HighCape Partners Management, L.P. is a venture capital firm specializing in growth equity investments. The firm seeks to invest in commercial stage life science companies that are in the early revenue or product launch stage and have a superior product that delivers value to the healthcare system and has a clear path to commercialization. HighCape Partners Management, L.P. is based in Baltimore, Maryland with an additional office in New York, New York.

Hard Yaka is an investment firm based in Crystal Bay, Nevada, founded in 2010. It pursues technology investments across private equity and venture capital, including buyouts and distressed opportunities, and it frequently seeks majority stakes. The firm focuses on digital transformation infrastructure, including portable identity, payments, marketplaces, and regtech, and also backs early-stage opportunities in exchange space startups, targeting verticals within exchange markets as well as horizontals such as messaging and payments across exchange sectors.

Columbia Partners Private Capital is an investment management and advisory firm and institutional investment team based in Bethesda, Maryland. Established in 2004, it sponsors private equity funds-of-funds and makes direct investments in privately held growth companies through separately managed accounts. The firm focuses on private market opportunities, including private equity, venture, and mezzanine debt, with over $440 million in assets under management. It serves institutional clients through a disciplined investment process aimed at delivering risk-adjusted returns.

Alexandria Venture Investments is the strategic venture capital arm of Alexandria Real Estate Equities. Based in Pasadena, California, it provides long-term strategic capital to innovative life sciences and technology companies developing breakthrough therapies, diagnostics, and platforms across biopharma, digital health, agrifoodtech, agtech, and related healthcare fields. Established in 1996, the firm invests across seed, early, and growth stages and leverages its deep industry knowledge, extensive investor networks, and scientific advisory resources to support portfolio companies. The unit also pursues opportunities at the intersection of real estate, life sciences, and technology, reflecting its parent company's sector focus and integrated ecosystem approach. It aims to help entities advance human health by partnering with founders to scale transformative technologies.

Syncom Venture Partners is a venture capital firm founded in 1977, based in Maryland, that provides early- and mid-stage investments in the media and communications sectors, including digital media, mobile technology, and web-based services.

Axcel Partners is a private equity and venture capital firm based in Stevenson, Maryland. The firm provides capital to companies across software, healthcare, information technology, and retail sectors.

SCI Ventures is a venture capital firm based in Baltimore, Maryland, established in 1972. The firm focuses on investing in early-stage companies primarily within the information technology, media, and telecommunications sectors. A significant aspect of SCI Ventures' mission is to support minority and women entrepreneurs in emerging domestic markets, which have historically been underfunded yet hold strong potential for innovation and growth. By concentrating on these underserved areas, SCI Ventures aims to create a competitive advantage in a dynamic business landscape, enabling both investors and entrepreneurs to capitalize on new opportunities. The firm emphasizes deploying capital in fragmented and evolving industries, thereby fostering sustainable development and positive economic impact.

GoodPaper Ventures is a venture capital firm based in Bethesda, Maryland, founded in 2021. The company specializes in providing private equity and investment management services, focusing on identifying and supporting innovative startups and growth-stage companies. By leveraging its expertise and resources, GoodPaper Ventures aims to foster entrepreneurship and drive economic growth through strategic investments in promising ventures.

Established in 2020, CoFactor Ventures is a venture capital firm headquartered in Baltimore, Maryland. It focuses on investing in tech startups at various stages of growth, from seed to later-stage companies.

Linden Lake Ventures is a Bethesda, Maryland-based early-stage biotechnology-focused venture capital firm founded in 2019. It invests in biotechnology companies developing molecular medicines and seeks to support pre-clinical and phase I technologies, with an emphasis on oncology, immunology, hepatology, cardiovascular, respiratory, neurology, and orphan diseases. The firm takes a hands-on approach to help portfolio companies advance their programs.

Founded in 2018, Singh Capital Partners is a private equity firm headquartered in Potomac, Maryland. It invests directly in venture capital, real estate, and growth equity opportunities.