RA Capital Management is a Boston-based investment firm focused on life sciences and healthcare. It provides venture and growth capital to public and private companies developing drugs, medical devices, diagnostics, and biotech tools, investing across stages from seed to IPO and follow-on financings. The firm often co-invests and takes board seats, pursuing opportunities in genetics, personalized medicine, cost-effective diagnostics, and nurse-enabled technologies. Its team combines expertise in biology, chemistry, and medicine with industry and business development experience. Founded in the early 2000s, RA Capital Management operates as an investment firm serving life sciences companies in the United States and Europe.

MassVentures is a Massachusetts-based venture capital firm that provides seed and early-stage funding to high-growth startups in the Massachusetts innovation economy, helping concepts move from idea to commercialization. Founded in 1978 as a quasi-public corporation by the Legislature of the Commonwealth, it is governed by an independent board and managed by experienced venture investors. The firm focuses on information technology, healthcare, cybersecurity, e-commerce, mobile, manufacturing, and robotics sectors in Massachusetts and operates accelerator programs to support portfolio companies. MassVentures leverages the state's entrepreneurial ecosystem, educated workforce, and centers of innovation to back early-stage ventures.

Polaris Partners is a venture capital firm based in Boston, Massachusetts, with offices in San Francisco. Founded in 1996, it targets investments in technology and life science companies across the United States and Europe, supporting startups from early formation to profitable growth. The firm's healthcare and biotechnology focus encompasses digital health, consumer-centric care delivery, patient-provider solutions, data science and analytics, as well as therapeutics, genomics and other life science platforms. It backs companies across business-to-business and business-to-consumer models, software as a service, healthcare information technology and health tech sectors, partnering with repeat entrepreneurs to build durable, scale-ready businesses.

Atlas Venture is an early-stage venture capital firm based in Cambridge, Massachusetts, specializing in life sciences and biotechnology. It supports the translation of high-impact scientific discoveries into medicines for patients through a seed-led venture creation strategy that identifies and builds scalable companies with strong scientific foundations. The firm combines experienced scientists and career venture capitalists to select compelling opportunities and realize value, emphasizing early-stage investments in life sciences and technology. Atlas Venture also maintains a multi-disciplinary team able to advance startups from concept to clinical development and commercial viability.

Morningside is a venture capital firm that funds companies with novel science and technology. It pursues a long-term, value-building approach and emphasizes ethical conduct. Based in the greater Boston area, the firm has experienced professionals with backgrounds across life sciences, digital health, artificial intelligence, materials and technology. Established in 1986 as a family office for the Chan family, Morningside has supported education, research and healthcare through charitable commitments to institutions such as the Harvard T.H. Chan School of Public Health, UMass Chan Medical School, and the MIT Morningside Academy for Design.

Founded in 2000, General Catalyst is a venture capital firm specializing in early-stage and growth equity investments. It focuses on accelerating ideas, careers, and companies towards success by providing ongoing momentum and mentorship based on deep experience.

Alumni Ventures Group is a venture capital firm based in Manchester, New Hampshire, established in 2013. It targets individual accredited investors who previously had limited access to venture capital by enabling alumni from top entrepreneurial schools to invest together in ventures led by fellow alumni. The firm backs companies with an alumni connection and an institutional lead investor with sector expertise. It offers focused funds that provide accredited investors access to a diversified venture portfolio by type, sector, stage, and geography. The organization engages in seed, early-stage, and late-stage pre-IPO investments across technology, consumer, financial services, health care, life sciences, and other sectors.

Founder Collective is a venture capital firm founded in 2009 and headquartered in Cambridge, Massachusetts, with an office in New York. It focuses on seed- and early-stage technology companies, investing primarily in software, information technology, and TMT sectors across the United States and globally. The firm was established by successful entrepreneurs who have built and exited companies, and it emphasizes a founder-friendly approach that aims to minimize capital requirements while supporting portfolio companies. Although industry-agnostic, Founder Collective prefers opportunities with an information technology component and pursues a hands-on, founder-oriented investment style through multiple co-managed funds.

New Enterprise Associates is a U.S.-based venture capital firm founded in 1977 and headquartered in Menlo Park, California. It invests in technology and healthcare companies across stages and geographies, supporting startups from seed to growth. The firm operates globally with investments in the United States, Asia, and Brazil among others, and has a long track record of portfolio IPOs and acquisitions. It has over $19 billion in cumulative committed capital.

Founded in 1986, ARCH Venture Partners is a venture capital firm specializing in investments that bring innovations in life sciences to market. It focuses on companies co-founded by scientists and entrepreneurs, with a strong track record in commercializing technologies from academic research institutions and national laboratories.

Accomplice is a Boston-based venture capital firm, with an additional office in San Francisco, specializing in seed and early-stage investments in technology startups. It primarily invests in companies focused on cybersecurity, eSports, data analytics, SMB class software, emerging hardware platforms, and marketplaces. Accomplice uniquely leverages community platforms like AngelList, Boston Syndicates, and Maiden Lane to empower and fund exceptional founders. With a majority of its investments in Boston and additional investments throughout the U.S., Canada, and the E.U., Accomplice is one of the most active early-stage firms in New England.

Founded in 1989, OrbiMed is a New York-based healthcare-dedicated investment firm managing approximately $5 billion in assets. It invests across the spectrum of healthcare companies, from private start-ups to large multinational corporations, focusing on biopharmaceuticals, life sciences, digital health, medical devices, and diagnostics sectors globally.

Flagship Pioneering is a Cambridge, Massachusetts-based venture creation firm that conceives, creates, resources, and grows first-in-category ventures to transform human health, sustainability, and related fields. It fuses scientific discovery with entrepreneurial leadership and professional capital to systematically build companies at the frontier of biology, engineering, medicine, energy, and beyond. The firm pursues ambitious, seemingly unreasonable propositions and steers them through an evolutionary development process to achieve transformational outcomes. By combining deep scientific insight with a structured, capital-backed platform, Flagship Pioneering accelerates progress in health technology, biotechnology and life sciences.

Mission BioCapital is a life science venture capital firm that backs early‑stage biotechnology companies. Based in Cambridge, Massachusetts, the firm draws on a leadership team with a long history of investing in and launching numerous early‑stage life science ventures. It operates in regions with strong research, discovery, and venture capital ecosystems and is known for providing deep expertise and active support to its portfolio companies.

Alexandria Venture Investments is the strategic venture capital arm of Alexandria Real Estate Equities. Based in Pasadena, California, it provides long-term strategic capital to innovative life sciences and technology companies developing breakthrough therapies, diagnostics, and platforms across biopharma, digital health, agrifoodtech, agtech, and related healthcare fields. Established in 1996, the firm invests across seed, early, and growth stages and leverages its deep industry knowledge, extensive investor networks, and scientific advisory resources to support portfolio companies. The unit also pursues opportunities at the intersection of real estate, life sciences, and technology, reflecting its parent company's sector focus and integrated ecosystem approach. It aims to help entities advance human health by partnering with founders to scale transformative technologies.

Spark Capital is a venture capital firm founded in 2005 and based in San Francisco with offices in Boston and New York. It invests in startups across the United States, spanning early-stage to growth opportunities, focusing on software, internet, media, consumer products and services, fintech, and information technology. The firm backs companies across sectors such as software-as-a-service, digital media, online platforms, and technology-driven consumer products, often supporting ventures at the intersection of entertainment and technology, e-commerce, and education technology. It typically makes investments from formative rounds up to larger growth rounds, and seeks to take a board seat in portfolio companies to help guide strategy and growth. Notable investments include Twitter, Discord, Anthropic, Cruise, Niantic, Oculus, Warby Parker, and Tumblr, illustrating a history of backing influential products and teams.

Casdin Capital is a New York-based investment firm focused on life sciences and healthcare. Founded in 2012 by Eli Casdin, the firm pursues private investments and research-driven opportunities across early- to late-stage companies in these sectors and is described as a venture capital firm and a registered investment adviser.

Matrix Partners is a San Francisco-based venture capital firm founded in 1977 that backs technology companies from idea through Series A and beyond. The firm focuses on software, AI, B2B, consumer, fintech, health tech, infrastructure, and related sectors, with a global footprint in the United States, India, and China. Its team comprises former founders and operators who work closely with portfolio companies and often join boards to support growth. Matrix Partners has backed a broad slate of successful companies in its decades-long history, including Apple, FedEx, Oculus, Zendesk, HubSpot, Canva, and Postmates, illustrating a pattern of helping developers and builders scale toward IPO or strategic exits. The firm's China arm extends its early-stage and growth investments in the Chinese market, reinforcing its international reach.

F-Prime Capital is a venture capital firm based in Cambridge, Massachusetts, affiliated with Fidelity Investments. It backs technology and life sciences entrepreneurs across the Americas, Europe, and Asia, drawing on Fidelity’s experience in funding early-stage companies. The firm focuses on sectors such as healthcare IT, medtech, therapeutics, fintech, frontier technologies, crypto, and broader information technology and technology-enabled services. With a global network of regional teams and affiliated funds, F-Prime provides domain expertise, strategic guidance, and industry connections to help founders build durable companies. It operates with a long-term, founder-centric approach, emphasizing value creation rather than external fundraising pressures.

Insight Partners is a global software investor that partners with growth-stage technology and software companies. Headquartered in New York City with offices in London, Tel Aviv, and Palo Alto, it concentrates on growth-stage software, internet, and data services, with notable focus areas including fintech, cybersecurity, AI/ML, DevOps, and healthcare. Founded in 1995, Insight Partners manages over $75 billion in regulatory assets under management and has invested in more than 750 companies worldwide, with over 55 portfolio companies achieving an IPO. The firm combines capital with hands-on, right-sized software expertise to support portfolio companies on their growth journey from first investment to IPO.

Eli Lilly and Company is a global pharmaceutical leader engaged in the discovery, development, manufacture, and sale of medicines. It operates facilities in the United States, Puerto Rico, and many other countries, with products sold in about 135 countries. The company focuses on therapies in neuroscience, cardiometabolic, cancer, and immunology, and also conducts research in animal health and animal agriculture. Lilly supports patient outcomes and education through a philanthropic foundation that funds programs in underserved communities, including STEM education in Indianapolis.

.406 Ventures is a Boston-based venture capital firm founded in 2005 that specializes in early-stage investing in enterprise information technology and services. The firm backs B2B software, cybersecurity, IT security and infrastructure, data and cloud technologies, technology-enabled business services, digital health, fintech, big data, and artificial intelligence, with a geographic focus on the Northeast United States. It often acts as lead or first institutional investor in de novo and early-stage rounds and supports portfolio companies through growth with follow-on investments. Typical initial commitments range from 2 to 5 million dollars, with total investments of about 6 to 10 million per company. The team emphasizes practical industry experience and networks to help companies scale.

Khosla Ventures is a California-based venture capital firm founded in 2004 by Vinod Khosla. The firm backs entrepreneurs with venture assistance, strategic guidance, and capital, focusing on bold, early-stage technology companies. It invests across sectors including AI, climate and sustainability, enterprise software, consumer technologies, fintech, digital health, medtech and diagnostics, therapeutics, and frontier technologies. Based in Menlo Park, the firm manages several funds to support startups across stages, prioritizing ventures that address large markets and have potential for significant social and economic impact.

SOSV is a global venture capital firm that supports early-stage startups through active, founder-first development programs. Its flagship initiatives HAX and IndieBio provide facilities, engineering resources, services, and laboratory equipment to accelerate product development and prepare companies for subsequent funding rounds. SOSV focuses on deep technology across health, environmental, and cross-border software sectors, aiming to solve human and planetary health challenges. In addition to providing capital, the firm emphasizes intensive programmatic support to help portfolio companies move quickly from seed toward growth, including customer acquisition and scale. The firm operates globally with a bias toward startups targeting rapid international expansion, especially in Asia, and tends to back a small cohort of high-potential companies to accelerate their progress and attract leading investors.

Pillar VC is a Boston-based venture capital firm established in 2016 that provides early capital and supports founders from inception through growth. It backs university spinouts led by first-time founders as well as serial entrepreneurs, aiming to build meaningful, scalable companies. The firm focuses on high-potential ventures across machine intelligence, AI, hardware, enterprise software, blockchain, quantum, digital health, and synthetic biology, and offers connections, coaching, and a community to help founders develop personally and professionally. Pillar VC is open to ventures of various types and emphasizes hands-on support and collaboration to accelerate progress.

SV Health Investors is a United States‑headquartered healthcare and life sciences venture capital and growth equity firm. Formerly known as SV Life Sciences, it supports entrepreneurs who create breakthrough companies and treatments across biotech, medical devices, and healthcare services. With offices in Boston, San Francisco and London, the firm has over two billion dollars of capital under management across seven private healthcare funds and more than two decades of activity in the US and Europe.

Highland Capital Partners is a Massachusetts-headquartered global venture capital firm that invests in seed, early and growth-stage consumer and enterprise technology companies. It maintains offices in Boston, Silicon Valley and Shanghai and has raised substantial capital and backed more than 225 companies across consumer and enterprise technology, supporting entrepreneurs with a focus on building lasting businesses. The firm also runs mentorship initiatives such as Summer@Highland. Its European presence, Highland Europe, founded in 2012, targets rapidly growing internet, mobile and software companies in Europe and works closely with the firm to pursue primary investments as well as acquisition finance or lead secondary transactions.

Third Rock Ventures is a Boston-based venture capital firm focused on investing in life sciences and healthcare companies in the United States. It backs startups developing medicines, diagnostics, medical devices, and related technologies with high growth potential, often providing substantial initial capital and strategic collaborations to advance development. The firm takes an active role in portfolio companies, frequently leading investment rounds and sometimes assuming key management positions, and collaborates with larger pharmaceutical and biotechnology companies. Since its founding in 2007, Third Rock has supported firms across biotechnology, therapeutics, oncology, medical devices, and data-driven life sciences, aiming to bring transformational healthcare innovations to market. The firm operates primarily from Boston with additional presence in San Francisco.

Bessemer Venture Partners is a long-established venture capital firm based in Redwood City, California, founded in 1911. The firm makes early-stage and growth investments across software, cloud, fintech, cybersecurity, artificial intelligence, biotech, healthcare, consumer services, marketplaces and other technology sectors, with global reach in the United States, Israel, Canada and India. It supports founders from seed to scale, providing strategic guidance and capital to build durable businesses. The firm has backed notable companies such as Pinterest, LinkedIn, Shopify, Yelp, Twilio, DocuSign and Wix, among others, reflecting a history of funding transformative technology companies. Bessemer Venture Partners operates as a registered investment adviser and pursues opportunities across information technology, hardware and services worldwide.

Hyperplane I, established in 2016, is a venture capital fund based in Boston, Massachusetts. It invests in exceptional founders developing machine intelligence and data companies, focusing on those that leverage AI, sensor technology, and cloud computing to tackle complex global challenges. The fund, managed by Hyperplane Venture Capital, was founded by Vivjan Myrto, John Murphy, and Brendan Kohler in 2015.

Deerfield Management is a New York-based investment firm established in 1994 that focuses on healthcare. It operates across investment, information, and philanthropy to support the healthcare ecosystem. The firm provides private and public investments and offers long-term, structured capital to both public and private healthcare companies. Its portfolio spans life sciences, medical devices, diagnostics, digital health, and health services. As an investment adviser, it seeks opportunities in healthcare products and services and aims to accelerate innovation by combining capital with industry insight.

Samsara BioCapital is a Palo Alto-based venture capital firm founded in 2016 that focuses on the healthcare sector, including biotechnology, pharmaceuticals, oncology, and life sciences. The firm seeks opportunities in the United States and provides investment advisory services as part of its activities, supporting companies developing innovative therapies and related technologies.

Underscore.VC is a Boston-based venture capital firm founded in 2015 that invests from seed through growth stages across the United States. It backs technology companies in sectors such as enterprise software, artificial intelligence and machine learning, fintech, insurtech, healthtech, e-commerce, the Internet of Things, and web3/blockchain, as well as data infrastructure, cloud software, and sensor-enabled industrial and home automation applications. With a focus on supporting bold entrepreneurs, the firm provides capital and strategic guidance to help startups accelerate product development and scale operations from pre-seed to Series A and beyond.

Launchpad Venture Group is an angel investment group and venture capital firm based in Boston, Massachusetts, that provides funding to early-stage technology and life sciences companies in the United States. It backs software, information technology, web, e-commerce, media and social media, mobile and wireless, medical devices, diagnostics, healthcare IT, networking and telecom, clean-tech, renewable energy and green-tech sectors. The firm seeks innovative startups addressing significant market opportunities and supports them with human and financial capital, leveraging its connections to customers, service providers, partners, and future sources of financing to help entrepreneurs build successful companies.

Battery Ventures is a technology-focused venture capital firm based in Boston, Massachusetts, founded in 1983. It invests in category-defining technology companies across sectors such as application software, infrastructure software, consumer internet and mobile services, industrial technologies, and life science tools. The firm supports growth across various stages and geographies, including the United States, Europe, and Israel, with emphasis on software, TMT, and related tech-enabled opportunities. As a registered investment adviser, Battery Ventures backs companies that build scalable platforms and transformative products, helping them compete in rapidly evolving markets.

Walnut Venture Associates is a Massachusetts-based venture capital firm focused on seed and early-stage information technology companies in New England. Based in Boston, it typically invests 250,000 to 1,000,000 per financing and seeks board representation in portfolio companies, providing active involvement and strategic support. The firm helps entrepreneurs refine business plans, assemble management teams, develop financing strategies, and establish strategic partnerships, leveraging the experience of a group of seasoned entrepreneurs and investors. One member often serves on the board, while the full capabilities of the group are available to portfolio companies.

Accel is a venture capital firm founded in 1983 by Arthur Patterson and Jim Swartz and headquartered in Palo Alto, California, with additional offices in San Francisco, London and Bangalore. It funds early- and growth-stage technology companies across software, cloud, SaaS, consumer, enterprise IT and related sectors, and it supports portfolio companies as they scale internationally. With a global network and more than three decades of experience, Accel seeks to help entrepreneurs build world-class, category-defining businesses. Notable investments include Atlassian, Facebook, Dropbox, Slack, Spotify, Etsy, Braintree, Cloudera and Qualtrics, among others. Accel operates multiple funds and programs to back companies at different stages, continuing a long track record of backing ambitious technology founders around the world.

Northpond Ventures is a science-driven venture capital firm headquartered in Bethesda, Maryland, with additional offices in San Francisco and Cambridge. Founded in 2018, the firm invests in life sciences and technology companies across biotechnology, diagnostics, digital health, environmental technologies, and related industries, including AI and machine learning-enabled therapeutics and medical technologies. It targets growth capital and early-stage opportunities, providing capital and strategic support to help scientific innovations reach the market. The firm operates as a registered investment adviser.

Venrock is a Palo Alto-based venture capital firm founded in 1969 as the venture capital arm of the Rockefeller family. It concentrates on seed and early-stage investments in technology and healthcare, with interests spanning information technology, life sciences and related sectors in the United States. The firm has supported a broad portfolio of companies, including Apple, Intel, Illumina, DoubleClick, Athenahealth, Gilead Sciences, Nest, SlideShare and Tudou. Venrock emphasizes long-term partnerships with entrepreneurs, combining hands-on venture support with rigorous data analysis to help startups grow into enduring companies.

LaunchCapital is a venture capital firm based in New Haven, Connecticut, that funds seed and early-stage startups. It focuses on technology, consumer, and medical sectors and targets commerce, marketplaces, media and entertainment, enterprise software and services, data, finance, healthcare, and emerging technologies. The firm maintains offices in Boston, New York, New Haven, and San Francisco and pursues opportunities for exponential growth by providing equity investments and other capital support to startups in the initial development stage. By helping founders scale from concept to revenue, LaunchCapital aims to optimize early-stage capital structures and support sustainable growth for portfolio companies.

Clean Energy Ventures is a Boston-based venture capital firm that invests in seed- and early-stage climate technology startups across North America, Europe and Israel. It focuses on advancing energy storage, grid connectivity, renewable energy production, clean transportation, and the water–energy nexus, supporting technologies and business models with scale potential to reduce greenhouse gas emissions. The firm backs companies that address climate change through market-driven approaches and seeks to accelerate commercialization of disruptive clean energy innovations.

Novo Holdings is a Danish holding and investment firm managing the assets of the Novo Nordisk Foundation. Established in 1999, it invests globally in life science companies at all stages of development, with a portfolio also including equities, bonds, real estate, and infrastructure.

Refactor Capital is a seed-stage venture capital firm based in Burlingame, California, founded in 2016. It backs early-stage startups across hard tech, biotechnology, health, software, pharmaceuticals, and related sectors. The firm is led by Zal Bilimoria, formerly a partner at a16z and a product manager at Google, Netflix, and LinkedIn, who acts as the sole decision maker and typically writes 1-2 million dollar checks in pre-seed and seed rounds.

Threshold Ventures, formerly Draper Fisher Jurvetson (DFJ), is a venture capital firm based in Palo Alto, California, that provides venture and growth capital to technology companies. It emphasizes a high-conviction investment approach and partners with entrepreneurs building software, consumer, enterprise, and healthcare technology ventures.

E14 Fund is a venture capital firm based in Cambridge, Massachusetts, that focuses on seed-stage investments in deep technology companies. It emphasizes commercializing MIT-originated ideas by providing capital, strategic guidance, and access to a broad network of advisors, including investors, industry experts, and entrepreneurs, to move technologies from lab to market and scale them for impact. The firm backs a diverse range of sectors, including biotechnology, smart cities, consumer electronics, and manufacturing, and seeks to connect founders with the resources needed to raise subsequent rounds and accelerate growth. Through its ecosystem, E14 Fund aims to bridge academia and industry, supporting entrepreneurs who leverage research and innovation to create scalable solutions.

Omega Funds is a Boston-based venture capital and private equity firm founded in 2004 that concentrates on early-stage investments in healthcare and biotech across North America and Western Europe, backing companies in life sciences, digital health, immunology, rare diseases, precision medicine, oncology, cybersecurity, and software-as-a-service.

Boston Seed Capital is a seed- and early-stage venture capital firm based in Massachusetts, with offices in Boston and Wellesley Hills. Founded in 2010, it focuses on funding internet-enabled and technology-driven startups across the United States, including consumer products, B2B software as a service, cybersecurity, e-commerce, big data, artificial intelligence and machine learning, marketplaces, and related services. The firm backs early-stage companies, often in information technology and SaaS, providing capital and strategic support to help companies scale from initial traction to growth.

Glasswing Ventures is a Boston-based venture capital firm founded in 2016 that specializes in early-stage investing in artificial intelligence, frontier technologies, and cybersecurity for enterprise applications. It backs pre-seed to startup and early-stage companies, primarily in North America, with a focus on the East Coast including Boston and New York areas. The firm leverages domain expertise and advisory networks to support founders who aim to transform markets with AI-powered and frontier tech solutions, particularly in enterprise software and security. Typical investments range from about 2 to 3 million dollars per company.

SR One is the corporate venture capital arm of GlaxoSmithKline, investing globally in early-stage life science companies pursuing innovative science with the potential to significantly improve medical care. Since 1985, SR One has invested about $800 million in more than 180 companies.

Mass General Brigham is an integrated academic health care system that connects a full continuum of care across medical facilities and services. It emphasizes patient care, research, and education while advancing innovative solutions in medicine. The organization serves as the principal teaching affiliate of Harvard Medical School and contributes to biomedical research.